미니돼지에서 자기 피부유래 전구세포와 탈회골 및 피브린 세케폴드를 이용한 하악골 골격손부의 골재생에 대한 연구
Mandibular bone regeneration using autogenous skin-derived precursor cells with a mixed demineralized bone and fibrin glue scaffold in miniature pigs
변준호, 김욱규, 김종렬, 박봉욱, 최문정, 최영진, 심경목,
소속 상세정보
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치과대학 구강악안면외과학교실
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치과대학 구강안면외과학교실
박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
최문정 ( Choi Mun-Jeoung ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
최영진 ( Choi Young-Jin ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
심경목 ( Shim Kyoung-Mok ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
KMID : 0360120090310030198
Abstract
Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal’s mandibular defects.
Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4 weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. 1x107 cells/100 microl of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold. In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies.
Results:Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4 weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the SKP-grafted group compared to the control group.
Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold can serve as a useful alternative to bone grafting technique.
키워드
Skin-derived precursor cells;In vitro osteogenesis;In vivo osteogeneis;Mandibular bone regeneration
원문 및 링크아웃 정보
등재저널 정보